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Identification of genes induced in proteoid roots of white lupin under nitrogen and phosphorus deprivation, with functional characterization of a formamidase

Permanent URL:
http://handle.nal.usda.gov/10113/54377
File:
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Abstract:
White lupin (Lupinus albus L.) is considered a model system for understanding plant acclimation to nutrient deficiency. It acclimates to phosphorus (P) and iron (Fe) deficiency by the development of short, densely clustered lateral roots called proteoid (or cluster) roots; proteoid-root development is further influenced by nitrogen (N) supply. In an effort to better understand proteoid root function under various nutrient deficiencies, we used nylon filter arrays to analyze 2,102 expressed sequence tags (ESTs) from proteoid roots of P-deficient white lupin. These have been previously analyzed for up-regulation in −P proteoid roots, and were here analyzed for up-regulation in proteoid roots of N-deprived plants. We identified a total of 19 genes that displayed up-regulation in proteoid roots under both P and N deprivation. One of these genes showed homology to putative formamidases. The corresponding open reading frame was cloned, overexpressed in E. coli, and the encoded protein was purified; functional characterization of the recombinant protein confirmed formamidase activity. Though many homologues of bacterial and fungal formamidases have been identified in plants, to our knowledge, this is the first report of a functional characterization of a plant formamidase.
Author(s):
Rath, Mousumi , Salas, Jay , Parhy, Bandita , Norton, Robert , Menakuru, Himabindu , Sommerhalter, Monika , Hatlstad, Greg , Kwon, Jaimyoung , Allan, Deborah L. , Vance, Carroll P. , Uhde-Stone, Claudia
Subject(s):
Lupinus albus , expressed sequence tags , gene expression regulation , genes , iron , nitrogen , nutrient deficiencies , open reading frames , phosphorus , recombinant proteins , roots
Note:
Includes references
Source:
Plant and soil 2010 Sept., v. 334, no. 1-2
Language:
English
Publisher:
Springer Netherlands
Year:
2010
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.