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Adaptation of lactic acid bacteria to butanol

Permanent URL:
http://handle.nal.usda.gov/10113/54062
File:
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Abstract:
Butanol can be produced biologically through fermentation of various substrates by Gram-positive Clostridium species. However, to profitably produce butanol at industrial scales, new microbial biocatalysts with increased tolerance to butanol are needed. In this study we report the isolation and selection of microbes to butanol. Ten strains, including Lactobacillus mucosae strains BR0605-3, BR0605-B15, BR0713-18, BR0713-20, BR0713-30, BR0713-33, Lactobacillus amylovorus strain NE-L 0206-19, Pediococcus parvulus NE-L 0206-31, Lactobacillus crispatus NE-L 0206-47, and Weissella confusa BR0216-18 were found capable of growth in 3–4% butanol after long term adaptation. These strains can be used to study tolerance mechanisms as well as identifying specific butanol stress response genes for development of butanol tolerant microbes.
Author(s):
Liu, Siqing , Bischoff, Kenneth M. , Leathers, Timothy D. , Qureshi, Nasib , Rich, Joseph O. , Hughes, Stephen R.
Subject(s):
Clostridium , Lactobacillus amylovorus , Lactobacillus crispatus , Lactobacillus mucosae , Pediococcus parvulus , Weissella confusa , alcoholic fermentation , butanol , enzymes , genes , genetic resistance , lactic acid bacteria , starter cultures , stress response
Note:
Includes references
Source:
Biocatalysis and agricultural biotechnology 2012 Jan., v. 1, no. 1
Language:
English
Year:
2012
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.