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The ectopic expression of the wheat Puroindoline genes increase germ size and seed oil content in transgenic corn

Permanent URL:
http://handle.nal.usda.gov/10113/49991
File:
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Abstract:
Plant oil content and composition improvement is a major goal of plant breeding and biotechnology. The Puroindoline a and b (PINA and PINB) proteins together control whether wheat seeds are soft or hard textured and share a similar structure to that of plant non-specific lipid-transfer proteins. Here we transformed corn (Zea mays L.) with the wheat (Triticum aestivum L.) puroindoline genes (Pina and Pinb) to assess their effects upon seed oil content and quality. Pina and Pinb coding sequences were introduced into corn under the control of a corn Ubiquitin promoter. Three Pina/Pinb expression positive transgenic events were evaluated over two growing seasons. The results showed that Pin expression increased germ size significantly without negatively impacting seed size. Germ yield increased 33.8% while total seed oil content was increased by 25.23%. Seed oil content increases were primarily the result of increased germ size. This work indicates that higher oil content corn hybrids having increased food or feed value could be produced via puroindoline expression.
Author(s):
Zhang, Jinrui , Martin, John M. , Beecher, Brian , Lu, Chaofu , Hannah, L. Curtis , Wall, Michael L. , Altosaar, Illimar , Giroux, Michael J.
Subject(s):
Triticum aestivum , gene expression , wheat germ , lipid bilayers , lipid content , wheat , corn oil , transgenic plants , corn , Zea mays , plant proteins , genetic transformation , promoter regions , ubiquitin , growing season , corn germ , yield components , hybrids
Format:
p. 353-365.
Note:
Includes references
Source:
Plant molecular biology 2010 Nov., v. 74, no. 4-5
Language:
English
Publisher:
Dordrecht : Springer Netherlands
Year:
2010
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.