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Microarray analysis reveals strategies of Tribolium castaneum larvae to compensate for cysteine and serine protease inhibitors

Permanent URL:
http://handle.nal.usda.gov/10113/49616
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Abstract:
The transcriptome response of Tribolium castaneum larvae to dietary protease inhibitors was evaluated by whole-genome microarray analysis. RNA was isolated from guts of larvae fed control diet (no inhibitor), or diets containing 0.1% E-64 (cysteine protease inhibitor), 5.0% soybean trypsin inhibitor (STI, serine protease inhibitor), or a combination of 0.1% E-64 and 5.0% STI. Data were analyzed by pairwise analysis, in which each inhibitor treatment group was compared to control, or ANOVA of all treatment groups. In pairwise analysis, the expression of only 253 genes was significantly altered (p < 0.05) in response to STI treatment, whereas E-64 and combination treatments resulted in 1574 and 1584 differentially regulated genes. The data indicate that treatments containing E-64, whether alone or in combination, significantly impacts gene expression in T. castaneum larvae. ANOVA analysis revealed 2175 genes differentially expressed in inhibitor-treated larvae compared to control (p < 0.05), including genes related to proteases that were mostly up-regulated, namely cathepsins B and L, chymotrypsins, and nonproteolytic cysteine cathepsin or serine protease homologs. Inhibitor treatments induced the differential expression of other gut-related genes, as well as genes encoding proteins of unknown function. These data suggest that T. castaneum larvae compensate for dietary cysteine protease inhibitors by altering large-scale gene expression patterns.
Author(s):
Oppert, Brenda , Elpidina, Elena N. , Toutges, Michelle , Mazumdar-Leighton, Sue
Subject(s):
microarray technology , Tribolium castaneum , larvae , cysteine proteinase inhibitors , proteinase inhibitors , transcriptome , genome , RNA , soybeans , gastrointestinal system , analysis of variance , gene expression , insect nutrition , adaptation
Format:
p. 280-287.
Note:
Includes references
Source:
Comparative biochemistry and physiology. Part D, Genomics and proteomics 2010 Dec., v. 5, no. 4
Language:
English
Year:
2010
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.