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Complete Transcriptome of the Soybean Root Hair Cell, a Single-Cell Model, and Its Alteration in Response to Bradyrhizobium japonicum Infection

Permanent URL:
http://handle.nal.usda.gov/10113/49205
Abstract:
Nodulation is the result of a mutualistic interaction between legumes and symbiotic soil bacteria (e.g. soybean [Glycine max] and Bradyrhizobium japonicum) initiated by the infection of plant root hair cells by the symbiont. Fewer than 20 plant genes involved in the nodulation process have been functionally characterized. Considering the complexity of the symbiosis, significantly more genes are likely involved. To identify genes involved in root hair cell infection, we performed a large-scale transcriptome analysis of B. japonicum-inoculated and mock-inoculated soybean root hairs using three different technologies: microarray hybridization, Illumina sequencing, and quantitative real-time reverse transcription-polymerase chain reaction. Together, a total of 1,973 soybean genes were differentially expressed with high significance during root hair infection, including orthologs of previously characterized root hair infection-related genes such as NFR5 and NIN. The regulation of 60 genes was confirmed by quantitative real-time reverse transcription-polymerase chain reaction. Our analysis also highlighted changes in the expression pattern of some homeologous and tandemly duplicated soybean genes, supporting their rapid specialization.
Author(s):
Libault, Marc , Farmer, Andrew , Brechenmacher, Laurent , Drnevich, Jenny , Langley, Raymond J. , Bilgin, Damla D. , Radwan, Osman , Neece, David J. , Clough, Steven J. , May, Gregory D. , Stacey, Gary
Subject(s):
Glycine max , root systems , plant response , transcriptome , gene expression regulation , bacterial diseases of plants , soil bacteria , symbionts , Bradyrhizobium japonicum , plant pathogenic bacteria , host-pathogen relationships
Format:
p. 541-552.
Note:
Includes references
Source:
Plant physiology 2010 Feb., v. 152, no. 2
Language:
English
Publisher:
American Society of Plant Biologists
Year:
2010
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF File]
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.