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Detection of Volatile Spoilage Metabolites in Fermented Cucumbers Using Nontargeted, Comprehensive 2-Dimensional Gas Chromatography-Time-of-Flight Mass Spectrometry (GC×GC-TOFMS)

Permanent URL:
http://handle.nal.usda.gov/10113/49076
File:
Download [PDF File]
Abstract:
A nontargeted, comprehensive 2-dimensional gas chromatography-time-of-flight mass spectrometry (GC×GC-TOFMS) method was developed for the analysis of fermented cucumber volatiles before and after anaerobic spoilage. Volatile compounds extracted by solid-phase microextraction were separated on a polyethylene glycol 1st-dimension column and 14% cyanopropylphenyl 2nd-dimension column. Among 314 components detected in fermented cucumber brine, 199 had peak areas with coefficients of variation below 30%. Peak identifications established by mass spectral library matching were 92% accurate based on 63 authentic standards. Analysis of variance of analytes’ log peak areas revealed 33 metabolites changed in concentration after spoilage (P < 0.05), including increases in acetic, propanoic, and butyric acids, n-propyl acetate, several alcohols, and a decrease in furfural. GC×GC-TOFMS with a nontargeted, semi-automated approach to data analysis made possible the separation, identification, and determination of differences in polar volatile components, facilitating the discovery of several metabolites related to fermented cucumber spoilage.
Author(s):
Johanningsmeier, Suzanne D. , McFeeters, Roger F.
Subject(s):
cucumbers , fermentation , fermented foods , food spoilage , anaerobes , biosynthesis , secondary metabolites , volatile organic compounds , polyethylene glycol , acetic acid , propionic acid , butyric acid , acetates , alcohols , capillary gas chromatography , mass spectrometry , culture filtrates
Format:
p. C168-C177.
Note:
Includes references
Source:
Journal of food science an official publication of the Institute of Food Technologists 2011 Jan-Feb, v. 76, no. 1
Language:
English
Year:
2011
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.