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Mate (Ilex paraguariensis St. Hilaire) saponins induce caspase-3-dependent apoptosis in human colon cancer cells in vitro

Permanent URL:
http://handle.nal.usda.gov/10113/49048
Abstract:
Saponins are naturally occurring metabolites associated with several health benefits. The objective was to quantify and purify saponins from mate dry leaves, and to assess their anti inflammatory and apoptotic mechanisms in human colon cancer cells in vitro. Matesaponins were extracted with methanol from dry leaves, partially purified and quantified. Leaves contained 10-15mg/g dry weight total saponins, predominantly matesaponins 1 and 2. HPLC and LC/ESI-MS-MS identified saponins in six preparative chromatographic fractions (A, B, C, D, E, and F). Major matesaponins were identified as 1 [M-H]⁻ =911 and 2 [M-H]⁻ =1057, with trace amounts of 3 [M-H]⁻ =1073, 4 [M-H]⁻ =1219, and 5 [M-H]⁻ =1383. Fractions D, E, and F significantly inhibited iNOS (IC₃₅ =36.3, 29.5, 43.7μM), PGE₂ (IC₃₅ =23.1, 22.3, 11.7μM) and COX-2 (IC₃₅ =45.7, 32.4, 17.0μM). Fraction F reduced nuclear translocation of nuclear factor-κB subunits p50 (49.8%) and p65 (49.0%) and induced apoptosis through suppression of Bcl-2 and increased Bax protein expressions and activated caspase-3 activity. Saponins in leaves of mate prevent inflammation and colon cancer in vitro.
Author(s):
Puangpraphant, Sirima , Berhow, Mark A. , Gonzalez de Mejia, Elvira
Subject(s):
yerba mate , herbal tea , Ilex paraguariensis , saponins , caspase-3 , enzyme activity , apoptosis , human cell lines , cell culture , colorectal neoplasms , in vitro studies , anti-inflammatory activity , medicinal plants
Format:
p. 1171-1178.
Note:
Includes references
Source:
Food chemistry 2011 Apr. 15, v. 125, no. 4
Language:
English
Publisher:
[Amsterdam]: Elsevier Science
Year:
2011
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF File]
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.