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Antisera-mediated in vivo reduction of Cry1Ac toxicity in Helicoverpa armigera

Permanent URL:
http://handle.nal.usda.gov/10113/48767
Abstract:
A functional assessment of Bacillus thuringiensis (Bt) toxin receptors in the midgut of lepidopteran insects will facilitate understanding of the toxin mode of action and provide effective strategies to counter the development of resistance. In this study, we produced anti-aminopeptidase (APN) and anti-cadherin sera with purified Cry1Ac toxin-binding APN or cadherin fragments from Heliocoverpa armigera. Antisera were evaluated for their effects on Cry1Ac toxicity through bioassays. Our results indicated that both the anti-APN and anti-cadherin sera reduced Cry1Ac toxicity in vivo, although cadherin antiserum reduced toxicity more than APN antiserum. These results suggest that both APN and cadherin are involved in Cry1Ac intoxication of H. armigera, evidence that the pore formation model may be representative of Cry1Ac toxin mode of action in this insect.
Author(s):
Liu, Chenxi , Gao, Yulin , Ning, Changming , Wu, Kongming , Oppert, Brenda , Guo, Yuyuan
Subject(s):
Helicoverpa armigera , insect control , biological control agents , Bacillus thuringiensis , bacterial insecticides , toxicity , bacterial proteins , crystal proteins , poisoning , biological control , defense mechanisms , insecticide resistance , binding capacity , cadherins , antiserum , membrane alanyl aminopeptidase , receptors , midgut , in vivo studies
Format:
p. 718-724.
Note:
Includes references
Source:
Journal of insect physiology 2010 July, v. 56, issue 7
Language:
English
Year:
2010
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF File]
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.