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Rapid evaluation of the antibiotic susceptibility of fuel ethanol contaminant biofilms

Permanent URL:
http://handle.nal.usda.gov/10113/48062
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Abstract:
Bacterial contaminants from commercial fuel ethanol production facilities were previously shown to form biofilms as mixed cultures under laboratory conditions. In this study, a rapid assay was developed to simultaneously compare isolates for their ability to form biofilms as pure cultures. A total of 10 strains were isolated from a dry-grind fuel ethanol plant that routinely doses with virginiamycin. These were identified by sequence analysis as six strains of Lactobacillus fermentum, two strains of L. johnsonii, and one strain each of L. mucosae and L. amylovorus. Isolates exhibited a range of susceptibility to virginiamycin in a planktonic assay, with MIC’s (minimum inhibitory concentration) of ⩽0.5–16μg/ml. Even though all strains were isolated from a mixed culture biofilm, they varied greatly in their ability to form biofilms as pure cultures. Surprisingly, growth as biofilms did not appear to provide resistance to virginiamycin, even if biofilms were grown for 144h prior to antibiotic challenge.
Author(s):
Joseph O. Rich , Timothy D. Leathers , Melinda S. Nunnally , Kenneth M. Bischoff
Note:
Batch:184 annotated on 2013-04-09T11:19:51Z
Source:
Bioresource technology 2011 January v.102 no.2
Language:
English
Publisher:
Elsevier Ltd
Year:
2011
Collection:
Journal Articles, USDA Authors, Peer-Reviewed