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In vitro antioxidant activity and inhibitory effect, on oleic acid-induced hepatic steatosis, of fractions and subfractions from oat (Avena sativa L.) ethanol extract
- Oats (Avena sativa L.) were extracted with 80% aqueous ethanol and the extract was successively isolated by liquid-liquid partition to yield n-hexane, ethyl acetate, n-butanol and water layers. Among them, the ethyl acetate (EA) layer exhibited the highest total phenolic content (TPC), the strongest DPPH radical-scavenging activity and an inhibitory effect on an oleic acid-induced (OA-induced) fatty liver model in vitro. Thus, it was further fractionated by a Sephadex LH-20 column into three subfractions (SF1-SF3). SF3 was the most active subfraction in all the assays above, the yield being 1.70% of the dry weight of the EA fraction. The major components in SF3 were identified as avenanthramides Bc, Bp and Bf by HPLC analysis, with contents of about 5.20%, 9.19% and 8.06% of the dry weight of SF3, respectively. Also, the avenanthramides Bc, Bp and Bf all had significant inhibitory effects on oleic acid-induced (OA-induced) fatty liver.
Cai, Shengbao , Huang, Chen , Ji, Baoping , Zhou, Feng , Wise, Mitchell L. , Zhang, Di , Yang, Peiying
oats , Avena sativa , food grains , supercritical fluid extraction , ethanol , phenolic compounds , plant extracts , antioxidants , antioxidant activity , free radical scavengers , oxidative stress , fatty liver , oleic acid , in vitro studies , inhibitors , fractionation , human cell lines , cell culture
- Includes references
- Food chemistry 2011 Feb. 1, v. 124, no. 3
- [Amsterdam]: Elsevier Science
Journal Articles, USDA Authors, Peer-Reviewed
- Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.