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White Lupin (Lupinus albus) response to phosphorus stress: evidence for complex regulation of LaSAP1

Permanent URL:
http://handle.nal.usda.gov/10113/46895
Abstract:
Proteoid roots are a unique adaptation that allow white lupin (Lupinus albus L. var Ultra) to survive under extreme phosphorus (P) deficient conditions. The cascade of events that signals P-deficiency induced gene expression in proteoid roots remains unknown. Through promoter::GUS analysis we showed that expression of acid phosphatase (LaSAP1) in P-deficient proteoid roots depends on DNA located from -465 bp to -345 bp 5' of the ATG start codon and that the P1BS (PHR1 Binding Site) element, located at -160 bp, also contributes regulatory control. DNA located within the -414 bp to -250 bp region of the LaSAP1 promoter was bound by nuclear proteins isolated from P-sufficient normal roots in electrophoretic mobility shift assays (EMSA), suggesting negative regulation. Competition experiments were performed with unlabeled oligonucleotides to further delineate the region of the LaSAP1 promoter bound by P-sufficient normal root nuclear proteins to a motif spanning -361 bp to -346 bp. The promoter motif characterized through EMSA spanning -361 bp to -345 bp was used as “bait” in a yeast one-hybrid (Y1H) experiment and 31 putative DNA binding proteins were isolated. Taken together, our results increase understanding of P-deficiency signaling by identifying regulatory regions and putative regulatory proteins for LaSAP1 expression.
Author(s):
Zinn, Kelly E. , Liu, Junqi , Allan, Deborah L. , Vance, Carroll P.
Subject(s):
Lupinus albus , plant response , root systems , soil nutrients , phosphorus , acid phosphatase , gene expression regulation , promoter regions , DNA-binding proteins , transcription factors , soil-plant interactions
Format:
p. 1-15.
Note:
Includes references
Source:
Plant and soil 2009 Sept., v. 322, no. 1-2
Language:
English
Publisher:
Dordrecht : Springer Netherlands
Year:
2009
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF File]
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.