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NALDC Record Details:
White Lupin (Lupinus albus) response to phosphorus stress: evidence for complex regulation of LaSAP1
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Proteoid roots are a unique adaptation that allow white lupin (Lupinus albus L. var Ultra) to survive under extreme phosphorus (P) deficient conditions. The cascade of events that signals P-deficiency induced gene expression in proteoid roots remains unknown. Through promoter::GUS analysis we showed that expression of acid phosphatase (LaSAP1) in P-deficient proteoid roots depends on DNA located from -465 bp to -345 bp 5' of the ATG start codon and that the P1BS (PHR1 Binding Site) element, located at -160 bp, also contributes regulatory control. DNA located within the -414 bp to -250 bp region of the LaSAP1 promoter was bound by nuclear proteins isolated from P-sufficient normal roots in electrophoretic mobility shift assays (EMSA), suggesting negative regulation. Competition experiments were performed with unlabeled oligonucleotides to further delineate the region of the LaSAP1 promoter bound by P-sufficient normal root nuclear proteins to a motif spanning -361 bp to -346 bp. The promoter motif characterized through EMSA spanning -361 bp to -345 bp was used as “bait” in a yeast one-hybrid (Y1H) experiment and 31 putative DNA binding proteins were isolated. Taken together, our results increase understanding of P-deficiency signaling by identifying regulatory regions and putative regulatory proteins for LaSAP1 expression.
Zinn, Kelly E.
Allan, Deborah L.
Vance, Carroll P.
Plant and soil 2009 Sept., v. 322, no. 1-2
Journal Articles, USDA Authors, Peer-Reviewed
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