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The usefulness of in vitro models to predict the bioavailability of iron and zinc: a consensus statement from the HarvestPlus expert consultation

Permanent URL:
http://handle.nal.usda.gov/10113/46036
Abstract:
A combination of dietary and host-related factors determines iron and zinc absorption, and several in vitro methods have been developed as preliminary screening tools for assessing bioavailability. An expert committee has reviewed evidence for their usefulness and reached a consensus. Dialyzability (with and without simulated digestion) gives some useful information but cannot predict the correct magnitude of response and may sometimes predict the wrong direction of response. Caco-2 cell systems (with and without simulated digestion) have been developed for iron availability, but the magnitude of different effects does not always agree with results obtained in human volunteers, and the data for zinc are too limited to draw conclusions about the validity of the method. Caco-2 methodologies vary significantly between laboratories and require experienced technicians and good quality cell culture facilities to obtain reproducible results. Algorithms can provide semi-quantitative information enabling diets to be classified as high, moderate, or low bioavailability. While in vitro methods can be used to generate ideas and develop hypotheses, they cannot be used alone for important decisions concerning food fortification policy, selection of varieties for plant breeding programs, or for new product development in the food industry. Ultimately human studies are required for such determinations.
Author(s):
Fairweather-Tait, S. , Lynch, S. , Hotz, C. , Hurrell, R. , Abrahamse, L. , Beebe, S. , Bering, S. , Bukhave, K. , Glahn, R. , Hambidge, M. , Hunt, Janet
Subject(s):
human nutrition , in vitro digestion , nutrient availability , iron , zinc , dietary minerals , iron absorption , intestinal absorption , dialysis , validity , cell culture , intestinal mucosa , algorithms
Format:
p. 371-374.
Note:
In the special issue: Estimating Iron and Zinc Bioavailability using In Vitro Models / edited by S. Fairweather-Tait and B. Lonnerdal.
Source:
International journal for vitamin and nutrition research 2005 Nov., v. 75, no. 6
Language:
English
Year:
2005
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF File]
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.