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Evaluation of enzymatic and non-enzymatic softening in low salt cucumber fermentations
- Retention of a firm, crisp fruit texture is a major consideration for pickled vegetables including pickles made from fermented cucumbers. It is known that cucumbers soften rapidly when fermented at low salt concentrations (<0.5 M) without added calcium. This study has shown that there is non-enzymatic softening in low salt fermentations because cucumbers soften even when heated sufficiently to inactivate pectinesterase and several glycosidases that can hydrolyse glycosidic linkages that are present in cell wall polysaccharides. Though pectinesterase activity declines and these glycosidases lose activity within the first week of fermentation there is generally greater loss of cucumber tissue firmness when enzymes are not inactivated by heat. While heating cucumbers prior to fermentation reduces softening during subsequent storage, a heat treatment after 2 weeks of fermentation does not reduce softening. This result suggested that the enzymatic reactions responsible for softening occur early in the fermentation process even though the softening does not become evident until later in the storage period. Despite the evidence of an enzymatic component of tissue softening in low salt cucumbers, softening could not be associated with specific enzymes.
Maruvada, Rashmi , McFeeters, Roger F.
brining , blanching , Cucumis sativus , fermented foods , cucumbers , fermentation , pickling , pickled vegetables , enzyme activity , salting , texture , hardness , heat inactivation , culture media , starter cultures , Lactobacillus plantarum , enzyme inactivation , pectinesterase , glycosidases , hydrogen bonding , hydrolysis , glycolysis , cell wall components , food quality , food safety
- Includes references
- International journal of food science & technology 2009 June, v. 44, no. 6
- Oxford, UK : Blackwell Publishing Ltd
Journal Articles, USDA Authors, Peer-Reviewed
- Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.