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Evaluation of quantitative PCR reference genes for gene expression studies in Tribolium castaneum after fungal challenge

Permanent URL:
http://handle.nal.usda.gov/10113/44808
Abstract:
To investigate gene expression in Tribolium castaneum exposed to Beauveria bassiana, reference genes for qPCR were evaluated. Of these, β-actin, α-tubulin, and RPS6 were not stable. The most stable were ribosomal protein genes, RPS3, RPS18, and RPL13a. Syntaxin1, syntaxin6, and E-cadherin may be appropriate for some experimental systems.
Author(s):
Lord, Jeffrey C. , Hartzer, Kris , Toutges, Michelle , Oppert, Brenda
Subject(s):
Tribolium castaneum , mycoses , infection , Beauveria bassiana , host-pathogen relationships , gene expression , messenger RNA , actin , tubulin , ribosomal proteins , genes
Format:
p. 219-221.
Note:
Includes references
Source:
Journal of microbiological methods 2010 Feb., v. 80, no. 2
Language:
English
Year:
2010
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF File]
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.