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Enumeration of Viable Listeria monocytogenes Cells by Real-Time PCR with Propidium Monoazide and Ethidium Monoazide in the Presence of Dead Cells

Permanent URL:
http://handle.nal.usda.gov/10113/44790
File:
Download [PDF File]
Abstract:
Propidium monoazide (PMA) and ethidium monoazide were used for enumeration of viable Listeria monocytogenes cells in the presence of dead cells. PMA had no antimicrobial effect on L. monocytogenes. Viable cell counts were linearly related to real-time PCR threshold cycle values for PMA-treated cells from planktonic and biofilm sources over a 4-log range.
Author(s):
Pan, Y. , Breidt, F. Jr.
Subject(s):
Listeria monocytogenes , polymerase chain reaction , viability , food microbiology , dyes , azides
Format:
p. 8028-8031.
Note:
Includes references
Source:
Applied and environmental microbiology AEM 2007 Dec. 15, v. 73, no. 24
Language:
English
Publisher:
American Society for Microbiology
Year:
2007
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.