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Spatial and temporal changes in the microbial community in an anaerobic swine waste treatment lagoon
- Microorganisms are central to both the beneficial (organic degradation, nutrient removal, biogas production) and detrimental (odor production, pathogen contamination) effects of swine waste storage systems. In this study, both quantitative (real-time polymerase chain reaction) and qualitative (denaturing gradient gel electrophoresis, cloning, sequence analysis) molecular analyses were used to track spatial and temporal changes in the microbial community of swine slurry from a 0.4 ha anaerobic lagoon. The lagoon, located in a region of western Kentucky which has a humid, subtropical environment, was sampled on a monthly basis (n = 10) over a period of one year at four different depths (top, 51 cm from the top, 152 cm from the top, and bottom >198 cm). The concentration and diversity of Bacteroides sp. was seasonal (up to 90% decrease between March and June). Hespellia sp. and other clostridial species, on the other hand, were endemic in the slurry (concentrations up to 1.0 x 10⁷ cells mL-1 slurry) regardless of time of the year or lagoon depth. Results suggest that there were seasonal effects on the microbial community in the swine lagoon, while the effect of depth was not as pronounced. Seasonal changes in the microbial community in stored wastes may be (directly or indirectly) correlated with changes in malodor emissions from lagoons.
Cook, Kimberly L. , Rothrock, Michael J. Jr. , Lovanh, Nanh , Sorrell, John K. , Loughrin, John H.
microorganisms , pig manure , wastewater treatment , waste lagoons , animal manure management , spatial variation , temporal variation , polymerase chain reaction , gel electrophoresis , molecular cloning , anaerobic conditions , slurries , humid tropics , Bacteroides , community structure , off odors , sequence analysis , Kentucky
- Includes references
- Anaerobe 2010 Apr., v. 16, no. 2
Journal Articles, USDA Authors, Peer-Reviewed
- Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.