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Evaluation of the waxy endosperm trait in proso millet (Panicum miliaceum)

Permanent URL:
http://handle.nal.usda.gov/10113/43943
File:
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Abstract:
The entire USDA-ARS maintained collection of 650 accessions of proso millet (Panicum miliaceum L.) was evaluated for the presence of accessions with waxy (amylose-free) endosperm starch. Six accessions, five of which derived from mainland China, were identified. Segregation ratios for waxy endosperm were evaluated in F₂ and F₃ populations derived from crosses between two waxy accessions, PI 436625 (Lung Shu 16) and PI 436626 (Lung Shu 18), and several wild-type accessions. The waxy trait was found to be under the control of duplicate recessive alleles at two loci, herein designated wx-1 and wx-2. Wild-type alleles at these loci were designated Wx-1 and Wx-2. Iodine-binding revealed a mean grain-starch amylose concentration of 3.5% in waxy lines and 25.3% in wild-type proso. Expression of the granule-bound starch synthase (waxy protein) in waxy lines was reduced to approximately one-tenth that of wild-type accessions. The waxy accessions identified now are available for the introgression of this trait into breeding lines adapted to the Great Plains of North America.
Author(s):
Graybosch, R.A. , Baltensperger, D.D.
Subject(s):
Panicum miliaceum , millets , endosperm , agronomic traits , amylose , inheritance (genetics)
Format:
p. 70-73.
Note:
Includes references
Source:
Plant breeding 2009 Feb., v. 128, no. 1
Language:
English
Publisher:
Oxford, UK : Blackwell Publishing Ltd
Year:
2009
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.