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Feasibility of colloidal silver SERS for rapid bacterial screening
- This study reports the feasibility of citrate-reduced colloidal silver surface-enhanced Raman scattering (SERS) for differentiating three important food borne pathogens, E. coli, Listeria, and Salmonella. FT-Raman and SERS spectra of both silver colloids and colloid-K3PO4 mixtures were collected and analyzed to evaluate the reproducibility and stability of silver colloids fabricated in a batch-production process. The results suggest that the reproducibility of the colloids over the batch process is high and that their binding effectiveness remains consistent over a 60-day storage period. Two specific SERS bands at 712 and 390 cm-1 were identified and used to develop simple 2-band ratios for differentiating E. coli-, Listeria-, and Salmonella-colloid mixtures with a 100% success. These results indicate that colloidal silver SERS technique may be a practical alternative method suitable for routine and rapid screening of E. coli, Listeria, and Salmonella bacteria.
Liu, Yongliang , Chao, Kuanglin , Nou, Xiangwu , Chen, Yud-Ren
food pathogens , bacterial contamination , mixed culture , microbial detection , silver , colloids , Escherichia coli , Listeria , Salmonella , screening , batch systems , food safety , bacterial adhesion , analytical methods , methodology
- Includes references
- Sensing and instrumentation for food quality and safety 2009 June, v. 3, no. 2
Journal Articles, USDA Authors, Peer-Reviewed
- Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.