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Simple Sequence Repeat Diversity of a Worldwide Collection of Puccinia triticina from Durum Wheat

Permanent URL:
http://handle.nal.usda.gov/10113/43023
File:
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Abstract:
Isolates of Puccinia triticina collected from durum wheat from Argentina, Chile, Ethiopia, France, Mexico, Spain, and the United States were analyzed with 11 simple sequence repeat (SSR) markers in order to determine the genetic relationship among isolates. These isolates also were compared with P. triticina isolates from common wheat from North America, and an isolate collected from Aegilops speltoides from Israel, to determine genetic relationships among groups of P. triticina found on different telial hosts. The large majority of isolates from durum wheat were identical for SSR markers or had <8% genetic dissimilarity, except for isolates from Ethiopia, which had 55% dissimilarity with respect to the other durum isolates. Isolates from common wheat had >70% genetic dissimilarity from isolates from durum wheat, and the isolate from A. speltoides was >90% dissimilar from all isolates tested. Analysis of molecular variance tests showed significant levels (P = 0.001) of genetic differentiation among regions and among isolates within countries. Isolates of P. triticina from durum wheat from South America, North America, and Europe were closely related based on SSR genotypes, suggesting a recent common ancestor, whereas P. triticina from Ethiopia, common wheat, and A. speltoides each had distinct SSR genotypes, which suggested different origins.
Author(s):
Ordoñez, M.E. , Kolmer, J.A.
Subject(s):
Triticum turgidum subsp. durum , durum wheat , rust diseases , Puccinia recondita , plant pathogenic fungi , pathogenicity , virulence , pathotypes , genetic relationships , genetic variation , microsatellite repeats , DNA profiling , center of origin , Argentina , Chile , Ethiopia , France , Mexico , Spain , United States
Format:
p. 574-583.
Note:
Includes references
Source:
Phytopathology 2007 May, v. 97, no. 5
Language:
English
Year:
2007
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.