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(-)-Gossypol Reduces Invasiveness in Metastatic Prostate Cancer Cells

Permanent URL:
http://handle.nal.usda.gov/10113/42619
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Abstract:
Background: Acquisition of metastatic ability by prostatic cancer cells is the most lethal aspect of prostatic cancer progression. (-)-Gossypol, a polyphenolic compound present in cottonseeds, possesses anti-proliferative and proapoptotic effects in various cancer cells. Materials and Methods: In this study, the differences between MAT-LyLu, rat prostate cancer cells, with a novel isolated subline from metastasized tumors in the lungs of MAT-LyLu-bearing Copenhagen rats (MLL cells) were compared with respect to cell growth and invasion. The effects of (-)-gossypol on cell viability, colony formation, invasive ability and cell migration in MAT-LyLu and MLL cells were also evaluated. Results: Results showed that MLL cells displayed higher growth ability, colony formation and aggressive penetration than those of MAT-LyLu cells. MLL cells possess lower protein expression of Bcl-xL and nm23-H1 than those of MAT-LyLu cells, implying differences in invasive ability. Moreover, (-)-gossypol treatment induced a dose-dependent inhibition of invasive activity and cell viability and reduced Bcl-2 and Bcl-xL proteins but induced nm23-H1 protein in both cell lines. Conclusion: These findings illustrated that (-)-gossypol reduced in vitro invasion of both the parental MAT-LyLu cells and the isolated MLL cells, suggesting that (-)-gossypol might serve as a chemotherapeutic and/or chemopreventive agent.
Author(s):
Huang, Yi-Wen , Wang, Li-Shu , Dowd, Michael K. , Wan, Peter J. , Lin, Young C.
Subject(s):
gossypol , prostatic neoplasms , chemical constituents of plants , metastasis , cottonseed , anticarcinogenic activity , apoptosis , animal disease models , rats , dose response , animal proteins , cell lines , cell growth
Format:
p. 2179-2188.
Note:
Includes references
Source:
Anticancer research 2009 June, v. 29, no. 6
Language:
English
Year:
2009
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.