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First infectious clone of the propagatively transmitted Oat blue dwarf virus
- Abstract::
- Oat blue dwarf virus (OBDV) is a small, phloem-limited marafivirus that replicates in its leafhopper vector. We have developed complete cDNA clones of OBDV from which infectious transcripts may be derived--the first such clones for any propagatively transmitted plant virus. Prior to clone construction, the reported sequences of the 5′ and 3′ ends were confirmed using 5′ RACE, primer extension, and ligation-anchored PCR. Using vascular puncture of maize seeds with capped transcripts, multiple clones were shown to be infectious at an average rate of 24.3% (range 14-36%). Aster leafhoppers successfully transmitted OBDV to oats and barley after feeding on detached, infected maize leaves. Proteins and RNAs consistent in size with those expected in OBDV infection were detected in young leaves via western and northern blotting, respectively. One construct, pOBDV-2r, was designated as the reference clone. An infectious clone of OBDV will be valuable in examining the interaction of this virus with both its insect and plant hosts.
- Author(s):
-
Edwards, Michael C. , Weiland, John J.
- Subject(s):
-
Avena sativa , oats , Hordeum vulgare , barley , Zea mays , corn , infection , Oat blue dwarf virus , clones , molecular cloning , complementary DNA , disease transmission , insect vectors , disease vectors , Macrosteles quadrilineatus
- Description:
- Includes references
- Source:
- Archives of virology 2010 Apr., v. 155, no. 4
- Language:
- English
- Year:
- 2010
- Publisher:
- Vienna : Springer Vienna
- Collection:
-
Journal Articles, USDA Authors, Peer-Reviewed
- File:
-
Download [PDF]
- Rights:
- Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
