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Molecular cloning and characterisation of heparanase mRNA in the porcine placenta throughout gestation

Permanent URL:
http://handle.nal.usda.gov/10113/42278
File:
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Abstract:
Heparanase (HPSE) is an endoglycosidase that specifically degrades heparan sulfate, which is an abundant glycosaminoglycan of the pig placenta. The aim of the present study was to clone cDNA encoding porcine HPSE and characterise the expression level and localisation of HPSE mRNA in porcine placentas throughout gestation. Placental tissues were collected from litters on Days 25, 45, 65, 85 and 105 of gestation. Three transcript variants similar to HPSE were identified in the pig placenta. In addition, the HPSE gene was mapped to pig chromosome 8 in close proximity to quantitative trait loci for litter size and prenatal survival. Real-time polymerase chain reaction and in situ hybridisation were used to characterise the expression of two HPSE variants, namely HPSE v1 and v2, in the pig placenta throughout gestation. The expression of HPSE v1 and v2 was elevated (P
Author(s):
Miles, Jeremy R. , Vallet, Jeffrey L. , Freking, Brad A. , Nonneman, Dan J.
Subject(s):
swine , placenta , pregnancy , molecular cloning , genes , extracellular matrix , gene expression , messenger RNA , glycosidases , glycosaminoglycans , chromosome mapping , quantitative trait loci
Format:
p. 757-772.
Note:
Includes references
Source:
Reproduction, fertility, and development 2009, v. 21, no. 6
Language:
English
Publisher:
Collingwood, Victoria: CSIRO Publishing
Year:
2009
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.