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Caenorhabditis elegans utilizes dauer pheromone biosynthesis to dispose of toxic peroxisomal fatty acids for cellular homoeostasis

Permanent URL:
http://handle.nal.usda.gov/10113/42082
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Abstract:
Caenorhabditis elegans excretes a dauer pheromone or daumone composed of ascarylose and a fatty acid side chain, the perception of which enables worms to enter the dauer state for long-term survival in an adverse environment. During the course of elucidation of the daumone biosynthetic pathway in which DHS-28 and DAF-22 are involved in peroxisomal β-oxidation of VLCFAs (very long-chain fatty acids), we sought to investigate the physiological consequences of a deficiency in daumone biosynthesis in C. elegans. Our results revealed that two mutants, dhs-28(tm2581) and daf-22(ok693), lacked daumones and thus were dauer defective; this coincided with massive accumulation of fatty acyl-CoAs (up to 100-fold) inside worm bodies compared with levels in wild-type N2 worms. Furthermore, the deficiency in daumone biosynthesis and the massive accumulation of fatty acids and their acyl-CoAs caused severe developmental defects with reduced life spans (up to 30%), suggesting that daumone biosynthesis is be an essential part of C. elegans homoeostasis, affecting survival and maintenance of optimal physiological conditions by metabolizing some of the toxic non-permissible peroxisomal VLCFAs from the worm body in the form of readily excretable daumones.
Author(s):
Joo, Hyoe-Jin , Yim, Yong-Hyeon , Jeong, Pan-Young , Jin, You-Xun , Lee, Jeong-Eui , Kim, Heekyeong , Jeong, Seul-Ki , Chitwood, David J. , Paik, Young-Ki
Subject(s):
Caenorhabditis elegans , pheromones , biosynthesis , metabolic detoxification , long chain fatty acids , lipid metabolism , oxidation , peroxisomes , abnormal development
Format:
p. 61-71.
Note:
Includes references
Source:
Biochemical journal 2009 Aug. 15, v. 422, pt. 1
Language:
English
Year:
2009
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.