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Relationships among calpastatin single nucleotide polymorphisms, calpastatin expression and tenderness in pork longissimus

Permanent URL:
http://handle.nal.usda.gov/10113/37274
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Abstract:
Genome scans in the pig have identified a region on chromosome 2 (SSC2) associated with tenderness. Calpastatin is a likely positional candidate gene in this region because of its inhibitory role in the calpain system that is involved in postmortem tenderization. Novel single nucleotide polymorphisms (SNP) in calpastatin were identified and used to genotype a population (n = 1042) of Duroc-Landrace-Yorkshire swine for association with longissimus lumborum slice shear force (SSF) measured at days 7 and 14 postmortem. Three genetic markers residing in the calpastatin gene were significantly associated with SSF (P < 0.0005). Haplotypes constructed from markers in the calpastatin gene were significantly associated with SSF (F-ratio = 3.93; P-value = 0.002). The levels of normalized mRNA expression of calpastatin in the longissimus lumborum of 162 animals also were evaluated by real-time RT-PCR and were associated with the genotype of the most significant marker for SSF (P < 0.02). This evidence suggests that the causative variation alters expression of calpastatin, thus affecting tenderness. In summary, these data provide evidence of several significant, publicly available SNP markers associated with SSF that may be useful to the swine industry for marker assisted selection of animals that have more tender meat.
Author(s):
Lindholm-Perry, A.K. , Rohrer, G.A. , Holl, J.W. , Shackelford, S.D. , Wheeler, T.L. , Koohmaraie, M. , Nonneman, D.
Subject(s):
swine , gene expression , single nucleotide polymorphism , calpastatin , genes , genetic markers , haplotypes , messenger RNA , meat tenderness , pork , meat quality
Format:
p. 713-721.
Note:
Includes references
Source:
Animal genetics 2009 Oct., v. 40, no. 5
Language:
English
Publisher:
Oxford, UK : Blackwell Publishing Ltd
Year:
2009
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.