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Functional Analysis of Pcipg2 From the Straminopilous Plant Pathogen Phytophthora capsici

Permanent URL:
http://handle.nal.usda.gov/10113/36493
Abstract:
Phytophthora capsici causes serious diseases in numerous crop plants. Polygalacturonases (PGs) are cell wall-degrading enzymes that play an important role in pathogenesis in straminopilous pathogens. To understand PGs as they relate to the virulence of P. capsici, Pcipg2 was identified from a genomic library of a highly virulent P. capsici strain. Pcipg2 was strongly expressed during symptom development after the inoculation of pepper leaves with P. capsici. The wild protein (PCIPGII) was obtained from the expression of pcipg2 and found that increasing activity of PGs in PCIPGII-treated pepper leaves was consistent with increasing symptom development. Asp residues in active sites within pcipg2 affected PCIPGII activity or its virulence on pepper leaves. Results show that pcipg2 is an important gene among pcipg genes, and illustrate the benefit of analyzing mechanisms of pathogenicity during the period of host/parasite interaction.
Author(s):
Sun, Wen Xiu , Jia, Yong Jian , Feng, Bao Zhen , O'Neill, Nichole R. , Zhu, Xiao Ping , Xie, Bing Yan , Zhang, Xiu Guo
Subject(s):
Phytophthora capsici , plant pathogenic fungi , polygalacturonase , gene expression regulation , pathogenicity , active sites , site-directed mutagenesis , enzyme activity , structure-activity relationships , host-pathogen relationships , peppers , Capsicum , reverse transcriptase polymerase chain reaction , Northern blotting , nucleotide sequences , amino acid sequences
Format:
p. 535-544.
Note:
Includes references
Source:
Genesis: the journal of genetics and development 2009 Aug., v. 47, issue 8
Language:
English
Year:
2009
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF File]
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.