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Conjugative Transfer of Plasmid-Located Antibiotic Resistance Genes Within the Gastrointestinal Tract of Lesser Mealworm Larvae, Alphitobius diaperinus (Coleoptera: Tenebrionidae)

Permanent URL:
http://handle.nal.usda.gov/10113/35236
File:
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Abstract:
The frequency of conjugative transfer of antimicrobial resistance plasmids between bacteria within the gastrointestinal tract of lesser mealworm larvae, a prevalent pest in poultry production facilities, was determined. Lesser mealworm larvae were exposed to a negative bacterial control, a donor Salmonella enterica serotype Newport strain, a recipient Escherichia coli, or both donor and recipient to examine horizontal gene transfer of plasmids. Horizontal gene transfer was validated post external disinfection, via a combination of selective culturing, testing of indole production by spot test, characterization of incompatibility plasmids by polymerase chain reaction, and profiling antibiotic susceptibility by a minimum inhibitory concentration (MIC) assay. Transconjugants were produced in all larvae exposed to both donor and recipient bacteria at frequencies comparable to control in vitro filter mating conjugation studies run concurrently. Transconjugants displayed resistance to seven antibiotics in our MIC panel and, when characterized for incompatibility plasmids, were positive for the N replicon and negative for the A/C replicon. The transconjugants did not display resistance to expanded-spectrum cephalosporins, which were associated with the A/C plasmid. This study demonstrates that lesser mealworm larvae, which infest poultry litter, are capable of supporting the horizontal transfer of antibiotic resistance genes and that this exchange can occur within their gastrointestinal tract and between different species of bacteria under laboratory conditions. This information is essential to science-based risk assessments of industrial antibiotic usage and its impact on animal and human health.
Author(s):
Crippen, Tawni L. , Poole, Toni L.
Subject(s):
Alphitobius diaperinus , Tenebrionidae , gastrointestinal system , larvae , plasmids , antibiotic resistance , poultry diseases , insect vectors , gene transfer , horizontal resistance , Salmonella enterica subsp. enterica serovar Newport , selective media , minimum inhibitory concentration , conjugation , Escherichia coli , food pathogens , disinfection , indoles
Format:
p. 907-915.
Note:
In the special issue: On-Farm Strategies to Reduce Foodborne Pathogen Contamination / edited by T.R. Callaway.
Source:
Foodborne pathogens and disease 2009 Sept., v. 6, no. 7
Language:
English
Year:
2009
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.