Search National Agricultural Library Digital Collections
Back to Search
NALDC Record Details:
Production and characterization of a broad-specificity polyclonal antibody for O,O-diethyl organophosphorus pesticides and a quantitative structure-activity relationship study of antibody recognition
Download [PDF File]
Polyclonal antibody (PAb) with broad-specificity for O,O-diethyl organophosphorus pesticides (OPs) against a generic hapten, 4-(diethoxyphosphorothioyloxy)benzoic acid, was produced. The obtained PAb showed high sensitivity to seven commonly used O,O-diethyl OPs in a competitive indirect enzyme-linked immunosorbent assay (ciELISA) using a heterologous coating antigen, 4-(3-(diethoxyphosphorothioyloxy)phenylamino)-4-oxobutanoic acid. The 50% inhibition value (IC50) was 348 ng mL-1 for parathion, 13 ng mL-1 for coumaphos, 22 ng mL-1 for quinalphos, 35 ng mL-1 for triazophos, 751 ng mL-1 for phorate, 850 ng mL-1 for dichlofenthion, and 1301 ng mL-1 for phoxim. The limit of detection (LOD) met the ideal detection criteria of all the seven OP residues. A quantitative structure-activity relationship (QSAR) model was constructed to study the mechanism of antibody recognition using multiple linear regression analysis. The results indicated that the frontier-orbital energies (energy of the highest occupied molecular orbital, E(HOMO), and energy of the lowest unoccupied molecular orbital, E(LUMO)) and hydrophobicity (log of the octanol/water partition coefficient, log P) were mainly responsible for the antibody recognition. The linear equation was log(IC50) = -63.274E(HOMO) + 15.985E(LUMO) + 0.556 log P - 25.015, with a determination coefficient (r2) of 0.908.
Beier, Ross C.
Analytica chimica acta 2009 Aug. 4, v. 647, issue 1
Journal Articles, USDA Authors, Peer-Reviewed
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Agricultural Research Service
Web Policies and Important Links