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Production and characterization of a broad-specificity polyclonal antibody for O,O-diethyl organophosphorus pesticides and a quantitative structure-activity relationship study of antibody recognition
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Polyclonal antibody (PAb) with broad-specificity for O,O-diethyl organophosphorus pesticides (OPs) against a generic hapten, 4-(diethoxyphosphorothioyloxy)benzoic acid, was produced. The obtained PAb showed high sensitivity to seven commonly used O,O-diethyl OPs in a competitive indirect enzyme-linked immunosorbent assay (ciELISA) using a heterologous coating antigen, 4-(3-(diethoxyphosphorothioyloxy)phenylamino)-4-oxobutanoic acid. The 50% inhibition value (IC50) was 348 ng mL-1 for parathion, 13 ng mL-1 for coumaphos, 22 ng mL-1 for quinalphos, 35 ng mL-1 for triazophos, 751 ng mL-1 for phorate, 850 ng mL-1 for dichlofenthion, and 1301 ng mL-1 for phoxim. The limit of detection (LOD) met the ideal detection criteria of all the seven OP residues. A quantitative structure-activity relationship (QSAR) model was constructed to study the mechanism of antibody recognition using multiple linear regression analysis. The results indicated that the frontier-orbital energies (energy of the highest occupied molecular orbital, E(HOMO), and energy of the lowest unoccupied molecular orbital, E(LUMO)) and hydrophobicity (log of the octanol/water partition coefficient, log P) were mainly responsible for the antibody recognition. The linear equation was log(IC50) = -63.274E(HOMO) + 15.985E(LUMO) + 0.556 log P - 25.015, with a determination coefficient (r2) of 0.908.
Beier, Ross C.
enzyme-linked immunosorbent assay
quantitative structure-activity relationships
Analytica chimica acta 2009 Aug. 4, v. 647, issue 1
Journal Articles, USDA Authors, Peer-Reviewed
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