Search National Agricultural Library Digital Collections
Back to Search
NALDC Record Details:
Influence of Temperature on Growth and Peak Oil Biosynthesis in a Carbon-Limited Medium by Pythium irregulare
Download [PDF File]
Kinetic analysis was investigated for a carbon-limited medium (C/N ratio = 5.0) supporting the growth of the 5,8,11,14,17-eicosapentaenoic acid (20:5; ω-3) (EPA)-accumulating fungal organism Pythium irregulare. The productivity and yield parameters at three temperatures, 14, 21, and 28°C, demonstrated growth-coupled synthesis for lipid-free biomass growth and lipid accumulation. For this system, the maximum specific growth rate and theoretical maximum biomass yield based on logistic growth kinetics were used to determine an activation energy of the growth process, E g, of 36.5 kJ mol⁻¹. At 14, 21, and 28°C, peak lipid yield occurred after culturing for 7, 4, and 3 days, respectively, with peak lipid yields of 8.14, 12.8, and 6.69 g lipid 100 g⁻¹ glucose. At these peak yields, the maximum lipid-free biomass productivity was achieved at the colder 14°C temperature as well as an increased concentration of EPA--10.9 wt%. Despite these enhancements, the maximum relative lipid production (P R₍FA/B₎) was achieved at 21°C--19.1%.
Cantrell, Keri B.
Walker, Terry H.
Journal of the American Oil Chemists' Society 2009 Aug., v. 86, no. 8
Journal Articles, USDA Authors, Peer-Reviewed
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Agricultural Research Service
Web Policies and Important Links