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Electrotransformation of Bacillus mojavensis with fluorescent protein markers
- Abstract::
- Gram-positive endophytic bacteria are difficult to transform. To study the interactions between Bacillus mojavensis and maize, a method was developed to transform the microbe by electroporation with three integration plasmids expressing green, cyan and yellow fluorescent proteins (GFP variants). GFP Transformations were verified by antibiotic selection, microscopy and amylase deficiency, based on incorporation of the plasmids through recombination with the amylase gene. Phenotypic expressions of endophytism and fungal antagonisms of the transformants were the same as wild type stains. This represents the first successful transformation of this endophytic species with GFP markers.
- Author(s):
-
Olubajo, Babatunde , Bacon, Charles W.
- Subject(s):
-
Bacillus mojavensis , endophytes , Zea mays , corn , genetic transformation , electroporation , plasmid vectors , green fluorescent protein , reporter genes
- Description:
- Includes references
- Source:
- Journal of microbiological methods 2008 Aug., v. 74, issue 2-3
- Language:
- English
- Year:
- 2008
- Collection:
-
Journal Articles, USDA Authors, Peer-Reviewed
- File:
-
Download [PDF]
- Rights:
- Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
