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A critical examination of the sodium dodecyl sulfate (SDS) sedimentation test for wheat meals

Permanent URL:
http://handle.nal.usda.gov/10113/30394
File:
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Abstract:
Sedimentation tests have long been used to characterise wheat flours and meals with the aim of predicting processing and end-product qualities. However, the use of the sodium dodecyl sulfate (SDS) sedimentation test for durum wheat (AACC International Approved Method 56-70) has not been characterised for hexaploid wheat varieties with a diverse range of protein quality and quantity. This paper reports the variation associated with important method parameters: sample weight, SDS concentration, technician, grinder and screen aperture (particle size). Sedimentation volumes were recorded every 5 min for 30 min and expressed as specific volume, i.e. sediment volume in mL g-1 meal. Ten diverse hexaploid wheat samples of markedly different protein quality and quantity were examined. The SDS sedimentation assay was shown to be highly robust and reproducible, with ANOVA (analysis of variance) model R2 values greater than 0.98 (individual time points). The procedure delineated soft and hard hexaploid wheat samples based on a combination of protein quantity and quality. Sample weight (if corrected to unit weight basis), recording time of at least 10 min, SDS stock concentration of at least 10 g L-1 (final), grinder type and screen aperture were minor sources of variation in SDS sedimentation volume relative to the effects due to differences among wheat samples. Interactions among ANOVA model terms were of relatively minor importance.
Author(s):
Morris, C.F. , Paszczynska, B. , Bettge, A.D. , King, G.E.
Subject(s):
wheat flour , wheat meal , food quality , food processing quality , durum wheat , protein composition , protein content , new methods , methodology , weight
Format:
p. 607-615.
Note:
Includes references
Source:
Journal of the science of food and agriculture 2007 Mar., v. 87, no. 4
Language:
English
Year:
2007
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.