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Microsatellite diversity of soybean genotypes differing in bean pod mottle virus leaf symptom

Permanent URL:
http://handle.nal.usda.gov/10113/29743
Abstract:
Bean pod mottle virus (BPMV) is a threat to soybean in most soybean [Glycine max (L.) Merr.] growing states of the USA. In the absence of complete resistance of soybean to BPMV, partial resistance can play an important role in reducing seed-yield and seed-quality losses from this virus. The objectives of this study were: (i) to evaluate plant introductions (PIs) for expression of leaf symptoms under greenhouse conditions following inoculation with BPMV, and (ii) to determine the genetic diversity among soybean genotypes with differences in leaf symptoms of BPMV. Large significant (P <= 0.001) differences in expression of BPMV leaf symptoms among genotypes in the greenhouse were detected. A genetic diversity study was conducted on 48 soybean genotypes differing in leaf symptoms of BPMV using 271 alleles amplified by 84 microsatellite primer pairs. The average polymorphism information content for the microsatellites was 0.53 and the average number of alleles per microsatellite was 3.23. The soybean genotypes clustered into distinct groups based on their country of origin and/or their BPMV leaf symptoms. Based on the microsatellite data, the genotypes were clustered into seven groups. Groups 1, 2 and 4 included genotypes from China only, group 3 included genotypes from the USA only and group 7 included genotypes from Japan only. Group 1 consisted of genotypes with low leaf symptoms of BPMV while group 6 was formed of genotypes with high leaf symptoms of BPMV.
Author(s):
Mian, M.A. Rouf , Kang, Sung-Taeg , Redinbaugh, Margaret G.
Subject(s):
Glycine max , genetic variation , introduced plants , genotype , Bean pod mottle virus , plant viruses , genetic resistance , disease resistance , genetic markers , alleles , genetic polymorphism , microsatellite repeats , leaves , signs and symptoms (plants) , provenance
Format:
p. 359-367.
Note:
Includes references
Source:
Canadian journal of plant science = Revue Canadienne de phytotechnie 2009 Mar., v. 89, no. 2
Language:
English
Year:
2009
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF File]
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.