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The selective Dectin-1 agonist, curdlan, induces an oxidative burst response in chicken heterophils and peripheral blood mononuclear cells

Permanent URL:
http://handle.nal.usda.gov/10113/27808
File:
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Abstract:
A critical component of host innate immunity is recognition of pathogen-associated molecular patterns (PAMPs) by pattern recognition receptors (PRRs). Dectin-1 is the primary PRR for exogenous β-glucan, a component of fungal and bacterial cell walls. A previous study conducted in our laboratory demonstrated that administration of β-glucan as a feed additive resulted in increased innate immune function of neonatal chickens, suggesting that chickens possess a Dectin-1-like β-glucan receptor. In the present study, we demonstrated that heterophils and peripheral blood mononuclear cells (PBMCs) from day-old chicks had a significant increase in the generation of reactive oxygen species (ROS) following stimulation with the Dectin-1 specific agonist, curdlan. Pretreatment of heterophils and PBMCs with laminarin, a β-glucan receptor blocking agent and specific inhibitor of Dectin-1 activity, significantly reduced the curdlan-induced ROS production. Together these data provide evidence for the first time of the presence of a functional Dectin-1-like β-glucan receptor in chicken heterophils and PBMCs.
Author(s):
Nerren, Jessica R. , Kogut, Michael H.
Subject(s):
curdlan , agonists , chickens , heterophils , immune response , beta-glucans , reactive oxygen species , leukocytes , receptors
Format:
p. 162-166.
Note:
Includes references
Source:
Veterinary immunology and immunopathology 2009 Jan. 15, v. 127, no. 1-2
Language:
English
Publisher:
Amsterdam: Elsevier
Year:
2009
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.