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Osmotic shock tolerance and membrane fluidity of cold-adapted Cryptococcus flavescens OH 182.9, previously reported as C. nodaensis, a biocontrol agent of Fusarium head blight

Permanent URL:
http://handle.nal.usda.gov/10113/26100
Abstract:
Cryptococcus flavescens (previously reported as C. nodaensis), a biological control agent of Fusarium head blight, has been previously shown to have improved desiccation tolerance after cold adaptation. The goal of the current study was to determine the effect of cold adaptation on the physicochemical properties of C. flavescens that may be responsible for its improved desiccation tolerance. The results show that cold adaptation improves liquid hyperosmotic shock tolerance and alters the temperature dependence of osmotic shock tolerance. Fluorescence anisotropy was used to characterize differences in the membrane fluidity of C. flavescens with and without cold adaptation. Force curves from atomic force microscopy showed a significant increase in the cell wall spring constant after cold adaptation. Cold adaptation of C. flavescens during culturing was shown to produce smaller cells and produced a trend towards higher CFU yields. These results suggest that cold adaptation significantly alters the membrane properties of C. flavescens and may be an effective method of improving the desiccation tolerance of microorganisms. In addition, we provide information on the correct naming of the isolate as C. flavescens.
Author(s):
Dunlap, Christopher A. , Evans, Kervin O. , Theelen, Bart , Boekhout, Teun , Schisler, David A.
Subject(s):
Cryptococcus (Hyphomycetes) , biological control agents , fungal antagonists , membrane fluidity , physicochemical properties , osmotic pressure , fungal anatomy , ultrastructure , fungal diseases of plants , blight , disease control , biological control , Fusarium
Format:
p. 449-458.
Note:
Includes references
Source:
FEMS yeast research 2007 May, v. 7, no. 3
Language:
English
Publisher:
Oxford, UK : Blackwell Publishing Ltd
Year:
2007
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF File]
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.