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Effect of selenium-enriched broccoli diet on differential gene expression in min mouse liver

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Multiple intestinal neoplasia (Min) mice are a good model for investigating the effects of dietary alterations in a genetic model for intestinal cancer. Previous studies have shown that selenium-enriched broccoli effectively reduces colon cancer susceptibility. Although colon cancer cells mainly metastasize to the liver, little is known about the effects of selenium-enriched broccoli on gene expression in mouse liver. To better understand the protective role for selenium-enriched broccoli in tumorigenesis, a gene profile of the mouse liver was analyzed. Mice were fed either 0.11 mg selenium/kg control diet or 2.1 mg selenium/kg selenobroccoli diets for 10 weeks. Use of mouse pathway finder-1 GEArrays revealed that selenium-enriched broccoli moderately increased ikBalphakappaB, hsp86, gadd45 gene transcripts. In addition, analysis of the binding of liver nuclear proteins to 32P-labeled probes demonstrated that selenium-enriched broccoli enhanced the binding of transcription factor p53, NFkappaB, AP-1 to their cis-acting elements. Collectively, these results suggest for the first time that selenium-enriched broccoli activates certain pro-apoptotic genes linked to p53, NFkappaB and stress signal pathways in response to "danger signals" such as tumorigenesis.
Zeng, H. , Davis, C.D. , Finley, J.W.
colorectal neoplasms , carcinogenesis , metastasis , apoptosis , nutritional intervention , enriched foods , broccoli , selenium , antioxidant activity , binding capacity , experimental diets , food intake , nutrient intake , mice , liver , transcription factors , messenger RNA , gene expression , transcriptional activation , DNA-binding proteins
p. 227-231.
Includes references
Journal of nutritional biochemistry Apr 2003. v. 14 (4)
Journal Articles, USDA Authors, Peer-Reviewed
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