Search National Agricultural Library Digital Collections

NALDC Record Details:

Planktonic and Biofilm Community Characterization and Salmonella Resistance of 14-Day-Old Chicken Cecal Microflora-Derived Continuous-Flow Cultures

Permanent URL:
http://handle.nal.usda.gov/10113/22637
File:
Download [PDF File]
Abstract:
This study evaluated the composition of gastrointestinal bacterial communities in birds during an age in which their susceptibility to Salmonella is highly diminished. One of the challenges to developing probiotics is to develop an efficacious culture of minimal diversity that includes bacteria that are vital contributors to protection from pathogens, but excludes unnecessary species. This study used in vitro continuous-flow culture techniques to test the ability of mixed bacterial cultures acquired from in vivo sources, to resist colonization by a marker Salmonella enterica serovar Typhimurium, and then characterized the constituents of both biofilm and planktonic communities by biochemical, phenotypic, and molecular methods. These cultures, initiated from 14-day-old chicks, were all able to restrict colonization by Salmonella in an average of 10 days. Eighteen species of bacteria from 10 different genera were characterized. However, each culture contained a mixture of only 11 species, which included lactic acid bacteria. Biofilms contained less than 50% of the species found in the planktonic communities. Although not adults, the diversity of microbes within the cecal cultures from 14-day-old birds represents a community complex enough to oppose colonization by a nonindigenous bacteria in vitro. These results describe bacterial mixtures containing less diversity than in previously described avian protective cultures.
Author(s):
Crippen, Tawni L. , Sheffield, Cynthia L. , Andrews, Kathleen , Dowd, Scot E. , Bongaerts, Roy J. , Nisbet, David J.
Subject(s):
biofilm , Salmonella enterica subsp. enterica serovar Typhimurium , intestinal microorganisms , disease resistance , chickens , cecum , chicks , probiotics , cell culture , bacterial colonization , microbial detection , phenotype , molecular biology
Format:
p. 1981-1987.
Note:
Includes references
Source:
Journal of food protection 2008 Oct., v. 71, no. 10
Language:
English
Year:
2008
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.