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Citrus Products Decrease Growth of E. coli O157:H7 and Salmonella Typhimurium in Pure Culture and in Fermentation with Mixed Ruminal Microorganisms In Vitro

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Orange peel and orange pulp are by-products that are included in feedlot and dairy cattle diets because of their low cost and high nutritional quality. The antimicrobial activity of citrus oils has been reported previously. The present study was carried out to determine whether these citrus by-products exert antimicrobial effects on Escherichia coli O157:H7 and Salmonella Typhimurium populations that are found in cattle gastrointestinal tracts. The growth of pure cultures (n = 3) of E. coli O157:H7 and Salmonella Typhimurium were reduced (p < 0.05) by addition of 2% (w/v) orange pulp and orange peel. Ruminal fluid was collected from cattle (n = 2) and E. coli O157:H7 or Salmonella Typhimurium were added. The addition of orange pulp and peel to in vitro mixed ruminal microorganism fermentations (n = 3) demonstrated that both orange pulp and peel reduced E. coli O157:H7 and Salmonella Typhimurium populations at least 2 log10 in mixed ruminal fluid fermentations. Addition of orange pulp reduced (p < 0.05) E. coli O157:H7 populations from 105 to 102 colony-forming units (CFU)/mL and Salmonella Typhimurium populations (p < 0.05) from 104 to 102 CFU/mL. These results indicate that orange pulp and/or peel included in ruminant diets could decrease ruminal populations of foodborne pathogenic bacteria. Further research is needed to determine whether the antimicrobial activity of orange products against E. coli O157:H7 or Salmonella Typhimurium is expressed in the lower gastrointestinal tract.
Callaway, T.R. , Carroll, J.A. , Arthington, J.D. , Pratt, C. , Edrington, T.S. , Anderson, R.C. , Galyean, M.L. , Ricke, S.C. , Crandall, P. , Nisbet, D.J.
food processing wastes , citrus peels , oranges , orange peels , orange pulp , waste utilization , antibacterial properties , nutritive value , feeds , cattle feeding , feedlots , beef cattle , dairy cattle , ruminant nutrition , in vitro culture , rumen fermentation , Escherichia coli O157:H7 , Salmonella typhimurium , bacterial contamination , food pathogens , shedding , rumen fluids , cross contamination , food contamination , plate count
p. 621-627.
Includes references
Foodborne pathogens and disease 2008 Oct., v. 5, no. 5
Journal Articles, USDA Authors, Peer-Reviewed
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