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Detection, Distribution, and Quantification of Silicon in Floricultural Crops utilizing Three Distinct Analytical Methods
- Silicon (Si) detection, distribution, and quantification in plants was compared using electron beam analysis (EBA; scanning electron microscopy coupled with energy dispersive X-ray analysis), colorimetric analysis, and inductively coupled plasma-optical emission spectroscopy (ICP-OES) in 14 economically important floriculture species. Using EBA, Si was identified most commonly around the base of trichomes and along the leaf margins. The ICP-OES processing and analysis for Si using sodium hydroxide (NaOH) resulted in damaged torches and microwavable Teflon® vessels that required expensive replacement at the end of each run, but this was not the case in the colorimetric method or with a potassium hydroxide (KOH)-based matrix in the ICP-OES. The results of these analyses suggest there is agreement between quantification methods, and EBA has a lower detection limit of about 300 mg kg-1 dry weight of Si. Several new floriculture species (zinnia, impatiens, verbena, and calibrachoa) were identified that take up and accumulate Si in significant concentrations.
Frantz, Jonathan M. , Locke, James C. , Datnoff, Lawrence , Omer, Medani , Widrig, Ann , Sturtz, Douglas , Horst, Leona , Krause, Charles R.
silicon , detection , quantitative analysis , scanning electron microscopy , energy-dispersive X-ray analysis , spectroscopy , colorimetry , plant tissues , leaves , trichomes , nutrient uptake , nutrient partitioning , new methods , floriculture crops , sodium hydroxide , potassium hydroxide
- Includes references
- Communications in soil science and plant analysis 2008 Oct., v. 39, no.17-18
Journal Articles, USDA Authors, Peer-Reviewed
- Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.