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Autofluorescence of Toxoplasma gondii and related coccidian oocysts

Abstract::
This is the first report of blue autofluorescence as a useful characteristic in the microscopic detection of Toxoplasma gondii, Hammondia hammondi, Hammondia heydorni, Neospora caninum, Besnoitia darlingi, and Sarcocystis neurona oocysts or sporocysts. This autofluorescence is of sufficient intensity and duration to allow identification of these oocysts from complex microscopic sample backgrounds. As with the autofluorescence of related coccidia, the oocysts glow pale blue when illuminated with an ultraviolet (UV) light source and viewed with the correct UV excitation and emission filter set.
Author(s):
Lindquist, H.D.A. , Bennett, J.W. , Hester, J.D. , Ware, M.W. , Dubey, J.P. , Everson, W.V.
Subject(s):
Toxoplasma gondii , Hammondia , Hammondia hammondi , Neospora caninum , Besnoitia , Sarcocystis neurona , oocysts , fluorescence , disease detection , pathogen identification , coccidiosis , Coccidia , sporocysts (Protozoa) , Hammondia heydorni
Description:
Includes references
Source:
Journal of parasitology 2003 Aug., v. 89, no. 4
Language:
English
Year:
2003
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF]   
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.