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Enteric bacteria of field-collected Colorado potato beetle larvae inhibit growth of the entomopathogens Photorhabdus temperata and Beauveria bassiana

Permanent URL:
http://handle.nal.usda.gov/10113/21084
Abstract:
The nematode Heterorhabditis marelatus fails to reproduce in the Colorado potato beetle, Leptinotarsa decemlineata, possibly due to interference from the enteric bacteria of the beetle. Specifically, the enteric bacteria inhibit the growth of Photorhabdus temperata, the enteric symbiont of the nematode, in vitro. However, previous work was based on a laboratory culture of L. decemlineata, and we wished to determine if similar bacteria were present in the field. Therefore, we cultured the enteric bacteria of fourth-instar larvae collected from the field at two locations in Maryland and Virginia. Representatives of the genera Pantoea, Enterobacter, Pseudomonas, Acinetobacter, Serratia, Stenotrophomonas, Curtobacterium, Bacillus, Lactococcus and Enterococcus were identified by sequencing of their 16S rDNA. Isolates belonging to the genera Pantoea, Enterobacter, Pseudomonas, Serratia and Bacillus inhibited the growth of P. temperata. A number of these isolates also inhibited the entomopathogenic fungus Beauveria bassiana in vitro.
Author(s):
Blackburn, Michael B. , Gundersen-Rindal, Dawn E. , Weber, Donald C. , Martin, Phyllis A.W. , Farrar, Robert R. Jr.
Subject(s):
Leptinotarsa decemlineata , larvae , intestinal microorganisms , bacteria , ribosomal DNA , sequence analysis , entomopathogenic bacteria , Photorhabdus , endosymbionts , entomopathogenic nematodes , Heterorhabditis marelatus , entomopathogenic fungi , Beauveria bassiana , microbial growth , Maryland , Virginia
Format:
p. 434-441.
Note:
Includes references
Source:
Biological control : theory and application in pest management 2008 Sept., v. 46, issue 3
Language:
English
Year:
2008
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF File]
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.