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Transcription Profiling of Soybean Nodulation by Bradyrhizobium japonicum

Permanent URL:
http://handle.nal.usda.gov/10113/20175
Abstract:
Legumes interact with nodulating bacteria that convert atmospheric nitrogen into ammonia for plant use. This nitrogen fixation takes place within root nodules that form after infection of root hairs by compatible rhizobia. Using cDNA microarrays, we monitored gene expression in soybean (Glycine max) inoculated with the nodulating bacterium Bradyrhizobium japonicum 4, 8, and 16 days after inoculation, timepoints that coincide with nodule development and the onset of nitrogen fixation. This experiment identified several thousand genes that were differentially expressed in response to B. japonicum inoculation. Expression of 27 genes was analyzed by quantitative reverse transcriptase-polymerase chain reaction, and their expression patterns mimicked the microarray results, confirming integrity of analyses. The microarray results suggest that B. japonicum reduces plant defense responses during nodule development. In addition, the data revealed a high level of regulatory complexity (transcriptional, post-transcriptional, translational, post-translational) that is likely essential for development of the symbiosis and adjustment to an altered nutritional status.
Author(s):
Brechenmacher, L. , Kim, M.Y. , Benitez, M. , Li, M. , Joshi, T. , Calla, B. , Lee, M.P. , Libault, M. , Vodkin, L.O. , Xu, D. , Lee, S.H. , Clough, S.J. , Stacey, G.
Subject(s):
Glycine max , soybeans , Bradyrhizobium japonicum , nodulation , transcriptomics , messenger RNA , complementary DNA , microarray technology , gene expression , temporal variation , reverse transcriptase polymerase chain reaction , resistance mechanisms , gene expression regulation , transcription (genetics) , translation (genetics)
Format:
p. 631-645.
Note:
Includes references
Source:
Molecular plant-microbe interactions : MPMI 2008 May, v. 21, no. 5
Language:
English
Year:
2008
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF File]
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.