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Effects of select nitrocompounds on in vitro ruminal fermentation during conditions of limiting or excess added reductant

Permanent URL:
http://handle.nal.usda.gov/10113/18881
File:
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Abstract:
Ruminal methane (CH₄) production results in the loss of up to 12% of gross energy intake and contributes nearly 20% of the United States' annual emission of this greenhouse gas. We report the effects of select nitrocompounds on ruminal fermentation after 22 h in vitro incubation (39°C) with or without additions of hydrogen (H₂), formate or both. In incubations containing no added reductant, CH₄ production was inhibited 41% by 2-nitro-1-propanol (2NPOH) and >97% by 3-nitro-1-propionic acid (3NPA), nitroethane (NE) and 2-nitroethanol (2NEOH) compared to non-treated controls and H₂ did not accumulate. With formate as the sole added reductant, nitro-treatment reduced CH₄ production by >99% and caused 42% to complete inhibition of formate catabolism compared to controls, and the accumulation of H₂ increased slightly. Nitro-treatment decreased CH₄ production 57-98% from that of controls when supplied H₂ or formate plus H₂. Formate catabolism was decreased 42-84% from that in controls by all nitro-treatments except 3NPA with both formate and H₂. Greater than 97% of the added H₂ was catabolized within controls; >84% was catabolized in nitro-treated incubations. Acetate, propionate and butyrate accumulations were unaffected by nitro-treatment irregardless of reductant; however, effects on ammonia and branched chain fatty acid accumulations varied. These results suggest that nitro-treatment inhibited formate dehydrogenase/formate hydrogen lyase and hydrogenase activity.
Author(s):
Anderson, Robin C. , Krueger, Nathan A. , Stanton, Thaddeus B. , Callaway, Todd R. , Edrington, Thomas S. , Harvey, Roger B. , Jung, Yong Soo , Nisbet, David J.
Subject(s):
nitrogen compounds , reduction , methane production , oxidoreductases , lyases , enzyme activity , volatile fatty acids , ammonia
Format:
p. 8655-8661.
Note:
Includes references
Source:
Bioresource technology 2008 Dec., v. 99, no. 18
Language:
English
Publisher:
[New York, NY]: Elsevier Ltd.
Year:
2008
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.