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Development of Sclerotinia Head Rot Resistant Germplasm Utilizing H. maximiliani and H. nuttallii

Permanent URL:
http://handle.nal.usda.gov/10113/16679
Abstract:
Interspecific hybridization of NMS HA 89 with Sclerotinia head rot resistant wild diploid perennial H. maximiliani and H. nuttallii accessions was successful using embryo rescue. A total of 162 F1 hybrid plants were obtained after rescuing 228 embryos from 70,500 pollinated florets. Most F1 plants had the expected 2n chromosome number of 34. A few F1 hybrids had a 2n=51 chromosome number which was assumed to have resulted from fertilization of normal gametes with unreduced gametes. Most F1 plants with the 2n=34 chromosome number had low pollen stainability of around 1%, and consequently low backcross seed set with only 85 seeds from 506 pollinated heads. Backcrosses of 2n=34 F1 plants with HA 441 produced BC1F1 progeny with 2n=34 or 35 chromosomes. Sib-pollination among heads with colchicine-induced chromosome doubling also had low seed set with 250 seeds from 425 pollinated heads producing amphiploids with 2n=62 to 68 chromosomes. Backcrossing of 2n=34 to 35 F1 plants with HA 441 is now in progress, as well as sib-pollination of the amphiploids for seed increase.
Author(s):
Jan, C.C. , Feng, J. , Seiler, G.J. , Rashid, K.Y.
Subject(s):
Sclerotinia , disease resistance , genetic resistance , plant rots , germplasm , interspecific hybridization , Helianthus annuus , Helianthus maximilianii , diploidy , hybrids , florets , pollination , chromosome number , pollen , backcrossing , seed set , colchicine , genes , lines
Format:
5 p.
Note:
Meeting held January 10-11, 2007, Fargo, ND.
Source:
Proceedings / Sunflower research workshop 2007, no. 29
Language:
English
Year:
2007
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF File]
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.