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Sulfite Analysis of Fruits and Vegetables by High-Performance Liquid Chromatography (HPLC) with Ultraviolet Spectrophotometric Detection

Permanent URL:
http://handle.nal.usda.gov/10113/15944
File:
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Abstract:
Free and total sulfite were analyzed in acidified vegetable products, instant mashed potatoes, and dried apples. Sulfite was separated by HPLC and quantified with a UV-vis detector. Resolution from components of food samples was achieved by varying the acid concentration of the eluant solution and by appropriate choice of the analytical wavelength. The minimum detectable levels for sulfite were 0.5 mg/L for a 10-cm analytical column and 1.5 mg/L for a 30-cm column. For analyses done with a 30-cm column, the coefficient of variation was <2% for analysis of free sulfite and total sulfite in acidified vegetables. For dried apples and instant potatoes, it ranged from 1 to 6.5%. The corresponding analytical errors were <4% and 1.2-5.6%, respectively, for the 10-cm column.
Author(s):
McFeeters, R.F. , Barish, A.O.
Subject(s):
sulfites , food additives , chemical residues , food contamination , high performance liquid chromatography , ultraviolet-visible spectroscopy , fruits (food) , vegetables , apples , dried fruit , potato flakes , hot peppers , sauces
Format:
p. 1513-1517.
Note:
Includes references
Source:
Journal of agricultural and food chemistry 2003 Mar., v. 51, no. 6
Language:
English
Year:
2003
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.