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Sequence analysis of the Lactobacillus plantarum bacteriophage phi JL-1

Permanent URL:
http://handle.nal.usda.gov/10113/15943
Abstract:
The complete genomic sequence of a Lactobacillus plantarum virulent phage ΦJL-1 was determined. The phage possesses a linear, double-stranded, DNA genome consisting of 36,677 bp with a G+C content of 39.36%. A total of 52 possible open reading frames (ORFs) were identified. According to N-terminal amino acid sequencing and bioinformatic analyses, proven or putative functions were assigned to 21 ORFs (41%), including 5 structural protein genes. The ΦJL-1 genome shows functionally related genes clustered together in a genome structure composed of modules for DNA replication, DNA packaging, head and tail morphogenesis, and lysis. This type of modular genomic organization was similar to several other phages infecting lactic acid bacteria. The structural gene maps revealed that the order of the head and tail genes is highly conserved among the genomes of several Siphoviridae phages, allowing the assignment of probable functions to certain uncharacterized ORFs from phage ΦJL-1 and other Siphoviridae phages.
Author(s):
Lu, Z. , Altermann, E. , Breidt, F. , Predki, P. , Fleming, H.P. , Klaenhammer, T.R.
Subject(s):
bacteriophages , Lactobacillus plantarum , starter cultures , cultured milk starters , genome , DNA , nucleotide sequences , open reading frames , viral proteins , amino acid sequences , genes
Format:
p. 45-54.
Note:
Includes references
Source:
Gene 2005 Mar. 28, v. 348
Language:
English
Year:
2005
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF File]
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.