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Morphological characterisation and genetic analysis of a bi-pistil mutant (bip) in Medicago truncatula Gaertn

Permanent URL:
http://handle.nal.usda.gov/10113/15578
File:
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Abstract:
A floral organ mutant was observed in transgenic Medicago truncatula Gaertn. plants that had two separate stigmas borne on two separate styles that emerged from a single superior carpel primordium. We propose the name bi-pistil, bip for the mutation. We believe this is the first report of such a mutation in this species. Genetic and molecular analyses of the mutant were conducted. The mutant plant was crossed to a mtapetala plant with a wild-type pistil. Expression of the mutant trait in the F₁ and F₂ generations indicates that the bi-pistil trait is under the control of a single recessive gene. Other modifying genes may influence its expression. The mutation was associated with the presence of a T-DNA insert consisting of the Alfalfa mosaic virus (AMV) coat protein gene in antisense orientation and the nptII selectable marker gene. It is suggested that the mutation is due to gene disruption because multiple copies of the T-DNA were observed in the mutant. The bi-pistil gene was found to be independent of the male-sterile gene, tap. This novel mutant may assist in understanding pistil development in legumes.
Author(s):
Nair, Ramakrishnan M. , Peck, David M. , Dundas, Ian S. , Samac, Deborah A. , Moore, Adam , Randles, John W.
Subject(s):
Medicago truncatula , transgenic plants , mutants , flowers , plant morphology , ultrastructure , plant genetics
Format:
p. 133-141.
Note:
Includes references
Source:
Sexual plant reproduction 2008 June, v. 21, no. 2
Language:
English
Publisher:
Berlin/Heidelberg : Springer-Verlag
Year:
2008
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.