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DNA diagnostics to identify internal feeders (Lepidoptera: Tortricidae) of pome fruits of quarantine importance

Permanent URL:
http://handle.nal.usda.gov/10113/1546
Abstract:
A diagnostic polymerase chain reaction (PCR) method is presented for differentiating among the North American internal apple-feeding pests codling moth, Cydia pomonella (L.); oriental fruit moth, Grapholita molesta (Busck); lesser appleworm, Grapholita prunivora (Walsh); and cherry fruitworm, Grapholita packardi Zeller. An approximately equal to 470-bp fragment of mitochondrial cytochrome oxidase subunit I (COI) was sequenced in three to six specimens of each species. Consistent and diagnostic differences were observed among the species in two regions of COI from which forward and reverse primers were designed to amplify a 112-116-bp segment of the gene. The primer sets were used to selectively amplify DNA from specimens of diverse geographic origin for each corresponding target species. Protocols were adapted for conventional and quantitative PCR, the latter being substantially faster. The method was validated as a decision-making tool for quarantine identifications for Mexico by representatives of their phytosanitary agency (Sanidad Vegetal). The method can facilitate identification of intercepted internal feeding Lepidoptera in apple and pear for many other importing nations.
Author(s):
Barcenas, N.M. , Unruh, T.R. , Neven, L.G.
Subject(s):
genes , cytochrome-c oxidase , pome fruits , pest identification , detection , mitochondrial DNA , polymerase chain reaction , nucleotide sequences , Cydia pomonella , Grapholita molesta , quarantine , Grapholita prunivora , Grapholita packardi , Mexico
Format:
p. 299-306.
Note:
Includes references
Source:
Journal of economic entomology 2005 Apr., v. 98, no. 2
Language:
English
Year:
2005
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF File]
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.