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Analysis of the Tribolium homeotic complex: insights into mechanisms constraining insect Hox clusters

Permanent URL:
http://handle.nal.usda.gov/10113/14251
Abstract:
The remarkable conservation of Hox clusters is an accepted but little understood principle of biology. Some organizational constraints have been identified for vertebrate Hox clusters, but most of these are thought to be recent innovations that may not apply to other organisms. Ironically, many model organisms have disrupted Hox clusters and may not be well-suited for studies of structural constraints. In contrast, the red flour beetle, Tribolium castaneum, which has a long history in Hox gene research, is thought to have a more ancestral-type Hox cluster organization. Here, we demonstrate that the Tribolium homeotic complex (HOMC) is indeed intact, with the individual Hox genes in the expected colinear arrangement and transcribed from the same strand. There is no evidence that the cluster has been invaded by non-Hox protein-coding genes, although expressed sequence tag and genome tiling data suggest that noncoding transcripts are prevalent. Finally, our analysis of several mutations affecting the Tribolium HOMC suggests that intermingling of enhancer elements with neighboring transcription units may constrain the structure of at least one region of the Tribolium cluster. This work lays a foundation for future studies of the Tribolium HOMC that may provide insights into the reasons for Hox cluster conservation.
Author(s):
Shippy, Teresa D. , Ronshaugen, Matthew , Cande, Jessica , He, JianPing , Beeman, Richard W. , Levine, Michael , Brown, Susan J. , Denell, Robin E.
Subject(s):
Tribolium castaneum , homeotic genes , sequence analysis , transcription (genetics) , embryogenesis , expressed sequence tags , mutation , transposons , enhancer elements , evolution , RNA interference , fluorescence in situ hybridization , genetic techniques and protocols
Format:
p. 127-139.
Note:
Includes references
Source:
Development genes and evolution 2008 Apr., v. 218, no. 3-4
Language:
English
Publisher:
Berlin/Heidelberg : Springer-Verlag
Year:
2008
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
File:
Download [PDF File]
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.