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Maize Centromere Mapping: A Comparison of Physical and Genetic Strategies

Permanent URL:
http://handle.nal.usda.gov/10113/13431
File:
Download [PDF File]
Abstract:
Centromere positions on 7 maize chromosomes were compared on the basis of data from 4 to 6 mapping techniques per chromosome. Centromere positions were first located relative to molecular markers by means of radiation hybrid lines and centric fission lines recovered from oat-maize chromosome addition lines. These centromere positions were then compared with new data from centric fission lines recovered from maize plants, half-tetrad mapping, and fluorescence in situ hybridizations and to data from earlier studies. Surprisingly, the choice of mapping technique was not the critical determining factor. Instead, on 4 chromosomes, results from all techniques were consistent with a single centromere position. On chromosomes 1, 3, and 6, centromere positions were not consistent even in studies using the same technique. The conflicting centromere map positions on chromosomes 1, 3, and 6 could be explained by pericentric inversions or alternative centromere positions on these chromosomes.
Author(s):
Okagaki, Ron J. , Jacobs, Morrison S. , Stec, Adrian O. , Kynast, Ralf G. , Buescher, Elizabeth , Rines, Howard W. , Vales, M. Isabel , Riera-Lizarazu, Oscar , Schneerman, Martha , Doyle, Greg , Friedman, Katherine L. , Staub, Rick W. , Weber, David F. , Kamps, Terry L. , Amarillo, Ina F.E. , Chase, Christine D. , Bass, Hank W. , Phillips, Ronald L.
Subject(s):
Zea mays , corn , centromeres , chromosome mapping , chromosome breakage , fluorescence in situ hybridization
Format:
p. 85-93.
Note:
Includes references
Source:
Journal of heredity 2008 Mar., v. 99, no. 2
Language:
English
Publisher:
The American Genetic Association
Year:
2008
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.